Protocols and tools

Protocols

To assess TP53 mutation status it is recommended to perform gene sequencing of all exons (or at least exons 4–10), including the splice junctions and completed with MLPA to detect large deletion/duplications. Immuno-histochemical analysis is not a good method for assessing TP53 mutation status, as it cannot distinguish between the various types of mutations, does not detect truncating mutations (false-negative results), and gives false-positive results.


Some example of sequencing protocols:



Useful tools