Protocols and tools

Protocols

The method we recommend to assess TP53 mutation status is to perform gene sequencing. Immuno-histochemical analysis is not a good method for assessing TP53 mutation status, as it cannot distinguish between the various types of mutations, does not detect truncating mutations (false-negative results), and gives false-positive results. Although most mutations cluster within exons 5-8, it is recommended to screen at least exons 4–10, including the splice junctions, so that less than 1% of mutations may be missed.

The standard sequencing procedure used at IARC for human TP53 gene is direct sequencing.

Protocols can be downloaded below:



Bioinformatics tools


Database tools