p53 Isoforms

Splicing variants of the human and mouse TP53 gene have been described in the eighties (Matlashewski et al., 1987, Flaman et al., 1996, Arai et al., 1986), but the existence and significance of these variants has only been recognized 20 years later (Courtois et al., 2002, Ghosh et al., 2004, Bourdon et al., 2005, Bourdon et al., 2007).

Several p53 human isoforms have been characterized that are produced by alternative splicing, use of alternative translation site or alternative promoter (see Joruiz & Bourdon, 2016; Marcel et al., 2011).

Most of these isoforms share a common DNA-binding domain with three different N-terminal domains (TA: entire transactivation domain; delta40: deleted from the first 39 residues; delta133: deleted from the first 132 residues) and/or three different C-terminal domains (alpha: entire oligomerisation domain; beta: 10 additional residues; gamma: 15 additional residues). By convention, TAp53alpha corresponds to the canonical p53 protein. In addition to these isoforms, deltap53 has been described that lacks residues from center of exon 7 to center of exon 9. See illustration below.

Current knowledge on the role and activities of these isoforms is still limited. In the IARC TP53 Database, all annotations and data are in relation to the full-lenght protein (TA isoform). New annotations have been introduced on the predicted impact of TP53 gene mutations on the protein status of the different isoforms (see here).





From Joruiz & Bourdon (CHS Prespectives, 2016). Top: TP53 gene and transcripts. Bottom: p53 protein isoforms.