Protocols and tools


To assess TP53 mutation status it is recommended to perform gene sequencing of all exons, including the splice junctions and completed with MLPA to detect large deletion/duplications. Immuno-histochemical analysis is not a good method for assessing TP53 mutation status, as it cannot distinguish between the various types of mutations, does not detect truncating mutations (false-negative results), and gives false-positive results (over-expressed WT protein).

Some example of sequencing protocols:

Recent developments on p53 antibodies

Other tools